Prof. Dr. Stefan Lorkowski

Funktionen

• Lehrstuhl für Biochemie und Physiologie der Ernährung

Forschungsgebiete

• Transfektion und Transduktion von Monozyten und Makrophagen
• Heterogenität von Monozyten und Plastizität von Makrophagen
• Molekulare Grundlagen der Atherosklerose und Inflammation
• Nutritive Modulation des Lipidstoffwechsels und zellulärer Lipidstoffwechsel/-transport
• Molekulare Wirkmechanismen von Nahrungsinhaltsstoffen

Publikationen (die 10 wichtigsten)

1. Rennert, K. Heisig, M. Groeger, M. Wallert, H. Funke, S. Lorkowski, O. Huber, A. S. Mosig, "Recruitment of CD16(+) monocytes to endothelial cells in response to LPS-treatment and concomitant TNF release is regulated by CX3CR1 and interfered by soluble fractalkine", Cytokine 2016, 83, 41-52.
2. Raasch, K. Rennert, T. Jahn, S. Peters, T. Henkel, O. Huber, I. Schulz, H. Becker, S. Lorkowski, H. Funke, A. Mosig, "Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions", Biofabrication 2015, 7, 015013.
3. Scherer, M.B. Maeß, S. Lindner, U. Garscha, C. Weinigel, S. Rummler, O. Werz, S. Lorkowski, "A procedure for efficient non-viral siRNA transfection of primary human monocytes using nucleofection", J. Immunol. Methods 2015, 422, 118-124.
4. B. Maeß, A. A. Keller, K. Rennert, A. Mosig, S. Lorkowski, "Optimization of the transfection of human THP-1 macrophages by application of Nunc UpCell technology", Anal. Biochem. 2015, 479, 40-42.
5. B. Maeß, B. Wittig, S. Lorkowski, "Highly efficient transfection of human THP-1 macrophages by nucleofection", J. Vis. Exp. 2014, 91, e51960.
6. Stiebing, C. Matthäus, C. Krafft, A. A. Keller, K. Weber, S. Lorkowski, J. Popp, "Complexity of fatty acid distribution inside human macrophages on single cell level using Raman micro-spectroscopy", Anal. Bioanal. Chem. 2014, 406, 7037-7046.
7. A. Keller, F. Mussbach, R. Breitling, P. Hemmerich, B. Schaefer, S. Lorkowski, S. Reissmann, "Relationships between cargo, cell penetrating peptides and cell type for uptake of non-covalent complexes into live cells", Pharmaceuticals 2013, 6, 184-203.
8. B. Maess, I. Buers, H. Robenek, S. Lorkowski, "Improved protocol for efficient nonviral transfection of premature THP-1 macrophages", Cold Spring Harbor Protoc. 2011, 2011, 508-511.
9. Robenek, I. Buers, O. Hofnagel, S. Lorkowski, N. J. Severs, "GFP-tagged proteins visualized by freeze-fracture immuno-electron microscopy: A new tool in cellular and molecular medicine", J. Cell. Mol. Med. 2009, 13, 1381-1390.
10. Schnoor, P. Cullen, J. Lorkowski, K. Stolle, H. Robenek, D. Troyer, J. Rauterberg, S. Lorkowski, "Production of type VI collagen by human macrophages: A new dimension in macrophage functional heterogeneity", J. Immunol. 2008, 180, 5707-5719.