Imaging mass spectrometry used in the JCSM allows the examination of tissue sections and thus the exact localization of thousands of proteins in an experiment. The advantage over previous proteomic studies is that the proteins need neither be labeled nor known beforehand. Using this method, the heterogeneity of tumors can be detected due to the different distribution of proteins but also of lipids. It is to be expected that with the help of this novel method also new tumor markers can be found.
D. Hölscher, S. Dhakshinamoorthy, T. Alexandrov, M. Becker, T. Bretschneider, A. Buerkert, A. C. Crecelius, D. D. Waele, A. Elsen, D. G. Heckel, H. Heklau, C. Hertweck, M. Kai, K. Knop, C. Krafft, R. K. Madulla, C. Matthäus, J. Popp, B. Schneider, U. S. Schubert, R. Sikora, A. Svatoš, R. Swennen, "Phenalenone-type phytoalexins mediate resistance of banana plants (musa spp.) to the burrowing nematode radopholus similis", Proc. Natl. Acad. Sci. U. S. A. 2014, in press.
A. C. Crecelius, T. Alexandrov, U. S. Schubert, "Application of matrix-assisted laser desorption / ionization mass spectrometry imaging to monitor surface changes of poly(styrene) films", Rapid Commun. Mass Spectrom. 2011, 25, 2809-2814.
J. T. Delaney, A. Urbanek, J. Perelaer, L. Wehder, A. C. Crecelius, F. v. Eggeling, U. S. Schubert, "Combinatorial optimization of multiple MALDI matrices on a single tissue sample using inkjet printing", ACS Comb. Sci. 2011, 13, 218-222.
L. Wehder, G. Ernst, A. C. Crecelius, O. Guntinas-Lichius, C. Melle, U. S. Schubert, F. von Eggeling, "Depicting the spatial distribution of proteins in human tumor tissue combining SELDI and MALDI imaging and immunohistochemistry", J. Histochem. Cytochem. 2010, 58, 929-937.