Prof. Dr. Stefan Lorkowski

Functions

• Professor of Biochemistry of Nutrition
• Head of Department "Biochemistry of Nutrition"

Research

• Transfection and transduction of monocytes and macrophages
• Heterozygosity of monocytes and plasticity of macrophages
• Molecular basis of atherosclerosis and inflammation
• Nutritive modulation of lipid metabolism and cellular lipid metabolism / transport
• Molecular mechanisms of action of nutritional constituents

Publications (the 10 most important)

1. Rennert, K. Heisig, M. Groeger, M. Wallert, H. Funke, S. Lorkowski, O. Huber, A. S. Mosig, "Recruitment of CD16(+) monocytes to endothelial cells in response to LPS-treatment and concomitant TNF release is regulated by CX3CR1 and interfered by soluble fractalkine", Cytokine 2016, 83, 41-52.
2. Raasch, K. Rennert, T. Jahn, S. Peters, T. Henkel, O. Huber, I. Schulz, H. Becker, S. Lorkowski, H. Funke, A. Mosig, "Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions", Biofabrication 2015, 7, 015013.
3. Scherer, M.B. Maeß, S. Lindner, U. Garscha, C. Weinigel, S. Rummler, O. Werz, S. Lorkowski, "A procedure for efficient non-viral siRNA transfection of primary human monocytes using nucleofection", J. Immunol. Methods 2015, 422, 118-124.
4. B. Maeß, A. A. Keller, K. Rennert, A. Mosig, S. Lorkowski, "Optimization of the transfection of human THP-1 macrophages by application of Nunc UpCell technology", Anal. Biochem. 2015, 479, 40-42.
5. B. Maeß, B. Wittig, S. Lorkowski, "Highly efficient transfection of human THP-1 macrophages by nucleofection", J. Vis. Exp. 2014, 91, e51960.
6. Stiebing, C. Matthäus, C. Krafft, A. A. Keller, K. Weber, S. Lorkowski, J. Popp, "Complexity of fatty acid distribution inside human macrophages on single cell level using Raman micro-spectroscopy", Anal. Bioanal. Chem. 2014, 406, 7037-7046.
7. A. Keller, F. Mussbach, R. Breitling, P. Hemmerich, B. Schaefer, S. Lorkowski, S. Reissmann, "Relationships between cargo, cell penetrating peptides and cell type for uptake of non-covalent complexes into live cells", Pharmaceuticals 2013, 6, 184-203.
8. B. Maess, I. Buers, H. Robenek, S. Lorkowski, "Improved protocol for efficient nonviral transfection of premature THP-1 macrophages", Cold Spring Harbor Protoc. 2011, 2011, 508-511.
9. Robenek, I. Buers, O. Hofnagel, S. Lorkowski, N. J. Severs, "GFP-tagged proteins visualized by freeze-fracture immuno-electron microscopy: A new tool in cellular and molecular medicine", J. Cell. Mol. Med. 2009, 13, 1381-1390.
10. Schnoor, P. Cullen, J. Lorkowski, K. Stolle, H. Robenek, D. Troyer, J. Rauterberg, S. Lorkowski, "Production of type VI collagen by human macrophages: A new dimension in macrophage functional heterogeneity", J. Immunol. 2008, 180, 5707-5719.