Prof. Dr. Michael Börsch

Functions

• Head of the interdisciplinary research group Microscopy Methodology

Research

• Single molecule-based fluorescence spectroscopic studies of the conformational dynamics of FoF1 ATP synthases from E. coli (biological nanomotor), ps time-resolved single-molecule FRET microscopy
• FLIM, ABELtrap, superresolution microscopy, black-lipid-membranes, hidden Markov models
• Conformational dynamics of individual membrane-bound transport ATPases (Pgp, KdpFABC, V-ATPase, A-ATP synthase), anisotropy microscopy, fluorescence correlation spectroscopy
• Ultrasensitive evidence for biochemical polymer degradation (nanocellulose)
• Local redox chemistry on nanoelectrodes (pH gradient for driving the ATP synthases), patch-clamp methods
• Fluorescence spectroscopic investigations of individual polymers (DNA block copolymers, poly-Bodipy dyes) and ultrastable perylenes

Publications (the 5 most important)

1. M. G. Düser, N. Zarrabi, D. J. Cipriano, S. Ernst, G. D. Glick, S.D. Dunn, M. Börsch, 36° step size of proton-driven c-ring rotation in F_oF₁-ATP synthase 2009 EMBO Journal 28, 2689-2696. (rated by Faculty-of-1000)
2. N. Zarrabi, S. Ernst, B. Verhalen, S. Wilkens, M. Börsch, Analyzing conformational dynamics of single P-glycoprotein transporters by Förster resonance energy transfer using hidden Markov models 2014 Methods 66, 168-179
3. H. Sielaff, M. Börsch, Twisting and subunit rotation in single F_OF₁-ATP synthase 2013 Phil. Trans. Royal Soc. B 368, 20120024
4. S. Ernst, M. G. Düser. N. Zarrabi, M. Börsch, Three-color Förster resonance energy transfer within single F_OF₁-ATP synthases: monitoring elastic deformations of the rotary double motor in real time 2012 J. Biomed. Opt. 17, 011004. (with cover page)
5. F. E. Alemdaroglu, S. C. Alexander, D. Ji, D. K. Prusty, M. Börsch, A. Herrmann, Poly(BODIPY)s: a new class of tunable polymeric dyes 2009 Macromolecules 42, 6529-6536.